On the Stability of a Polling System with an Adaptive Service Mechanism

We consider a single-server cyclic polling system with three queues where the server follows an adaptive rule: if it finds one of queues empty in a given cycle, it decides not to visit that queue in the next cycle. In the case of limited service policies, we prove stability and instability results under some conditions which are sufficient but not necessary, in general. Then we discuss open problems with identifying the exact stability region for models with limited service disciplines: we conjecture that a necessary and sufficient condition for the stability may depend on the whole distributions of the primitive sequences, and illustrate that by examples. We conclude the paper with a section on the stability analysis of a polling system with either gated or exhaustive service disciplines.Comment: 16 page

A polling system whose stability region depends on a whole distribution of service times

We present an example of a single-server polling system with two queues and an adaptive service policy where the stability region depends on the expected values of all the primitives and also on a certain exponential moment of the service-time distribution in one of the queues. The latter parameter can not be determined, in general, in terms of any finite number of power moments. It follows that the fluid approximation approach may not be an appropriate tool for the stability study of this model.Comment: 6 page

Performance analysis of polling systems with retrials and glue periods

We consider gated polling systems with two special features: (i) retrials, and (ii) glue or reservation periods. When a type-$i$ customer arrives, or retries, during a glue period of station $i$, it will be served in the next visit period of the server to that station. Customers arriving at station $i$ in any other period join the orbit of that station and retry after an exponentially distributed time. Such polling systems can be used to study the performance of certain switches in optical communication systems. For the case of exponentially distributed glue periods, we present an algorithm to obtain the moments of the number of customers in each station. For generally distributed glue periods, we consider the distribution of the total workload in the system, using it to derive a pseudo conservation law which in its turn is used to obtain accurate approximations of the individual mean waiting times. We also consider the problem of choosing the lengths of the glue periods, under a constraint on the total glue period per cycle, so as to minimize a weighted sum of the mean waiting times

Aggregation kinetics of human mesenchymal stem cells under wave motion

Human mesenchymal stem cells (hMSCs) are a primary candidate in cell therapy and regenerative medicine to treat a wide range of diseases in clinical trials. Recent studies showed that hMSC have innate ability to self-assemble into three-dimensional (3D) aggregates that enhances their therapeutic functions with augmented multi-lineage differentiation potential, migration ability, secretion of anti-inflammatory and angiogenic factors, and resistance to ischemic conditions post-transplantation. To date, many laboratory methods have been developed for hMSC aggregation, including hanging drops, centrifugation with microfabricated surface, cell suspension on a low attachment surface, thermal lifting, and microfluidic technologies. However, these methods have limited scalability and/or poor control in aggregate size, and cannot meet the required production in clinical trials. The objective of current study is to investigate the conditions for the scalable production of hMSC aggregates in non-adherent plates under wave motion. The repeated back and forth wave motion induced by rocking provides mixing of bulk medium under low shear stress that facilitates cell-cell collisions and subsequent aggregation. Our results showed that aggregate size can be controlled by adjusting the combination of rocking angle (3˚, 6˚, and 9˚) and rocking speed (10, 15, and 20 rpm). To quantify the impact of fluid shear stress on aggregation kinetics, simulation of shear stress distribution by COMSOL Multiphysics® showed a time-dependent oscillatory function under different rocking condition. In addition, an inverse correlation between aggregate size and maximum shear stress was observed and that both can be regressed by a two-variable linear regression of rocking angle and rocking speed. In the regression, the coefficient of rocking angle is much higher than that of rocking speed, revealing that rocking angle has a more significant effect than rocking speed on both aggregate size and shear stress. In addition to fluid shear stress, the effects of cell binding molecules, the frequency of cell-cell collision, and the extension of cultivation time on aggregate size distribution were also investigated. Analysis of the therapeutic functional supported that hMSCs derived from engineered aggregates in the wave motion system have enhanced their therapeutic properties compared to those from monolayer culture

RNase E and the High-Fidelity Orchestration of RNA Metabolism.

The bacterial endoribonuclease RNase E occupies a pivotal position in the control of gene expression, as its actions either commit transcripts to an irreversible fate of rapid destruction or unveil their hidden functions through specific processing. Moreover, the enzyme contributes to quality control of rRNAs. The activity of RNase E can be directed and modulated by signals provided through regulatory RNAs that guide the enzyme to specific transcripts that are to be silenced. Early in its evolutionary history, RNase E acquired a natively unfolded appendage that recruits accessory proteins and RNA. These accessory factors facilitate the activity of RNase E and include helicases that remodel RNA and RNA-protein complexes, and polynucleotide phosphorylase, a relative of the archaeal and eukaryotic exosomes. RNase E also associates with enzymes from central metabolism, such as enolase and aconitase. RNase E-based complexes are diverse in composition, but generally bear mechanistic parallels with eukaryotic machinery involved in RNA-induced gene regulation and transcript quality control. That these similar processes arose independently underscores the universality of RNA-based regulation in life. Here we provide a synopsis and perspective of the contributions made by RNase E to sustain robust gene regulation with speed and accuracy.Wellcome Trus

Progress in the use of ionic liquids as electrolyte membranes in fuel cells

This work provides a critical review of the progress in the use of Room Temperature Ionic Liquids (RTILs) as Proton Exchange Membrane (PEM) electrolytes in Fuel Cells (FCs). It is well-known that for an efficient early commercialisation of this technology it is necessary to develop a proton exchange membrane with high proton conductivity without water dependency capable of working at temperatures above 100 °C. The use of ionic liquids as electrolytes in electrochemical devices is an emerging field due to their high conductivity, as well as their thermal, chemical and electrochemical stability under anhydrous conditions. This paper attempts to give a general overview of the state-of-the-art, identifies the key factors for future research and summarises the recent progress in the use of ionic liquids as an innovative type of PEMs.This research was supported by the Ministry of Education, Culture and Sport under the Project CTQ2012-31639 (MINECO, SPAIN-FEDER2001–2013)

Differential Muc2 and Muc5ac secretion by stimulated guinea pig tracheal epithelial cells in vitro

BACKGROUND: Mucus overproduction is a characteristic of inflammatory pulmonary diseases including asthma, chronic bronchitis, and cystic fibrosis. Expression of two mucin genes, MUC2 and MUC5AC, and their protein products (mucins), is modulated in certain disease states. Understanding the signaling mechanisms that regulate the production and secretion of these major mucus components may contribute significantly to development of effective therapies to modify their expression in inflamed airways. METHODS: To study the differential expression of Muc2 and Muc5ac, a novel monoclonal antibody recognizing guinea pig Muc2 and a commercially-available antibody against human MUC5AC were optimized for recognition of specific guinea pig mucins by enzyme-linked immunosorbent assay (ELISA), Western blot, and immunohistochemistry (IHC). These antibodies were then used to analyze expression of Muc2 and another mucin subtype (likely Muc5ac) in guinea pig tracheal epithelial (GPTE) cells stimulated with a mixture of pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and interferon- γ (IFN-γ)]. RESULTS: The anti-Muc2 (C4) and anti-MUC5AC (45M1) monoclonal antibodies specifically recognized proteins located in Muc2-dominant small intestinal and Muc5ac-dominant stomach mucosae, respectively, in both Western and ELISA experimental protocols. IHC protocols confirmed that C4 recognizes murine small intestine mucosal proteins while 45M1 does not react. C4 and 45M1 also stained specific epithelial cells in guinea pig lung sections. In the resting state, Muc2 was recognized as a highly expressed intracellular mucin in GPTE cells in vitro. Following cytokine exposure, secretion of Muc2, but not the mucin recognized by the 45M1 antibody (likely Muc5ac), was increased from the GPTE cells, with a concomitant increase in intracellular expression of both mucins. CONCLUSION: Given the tissue specificity in IHC and the differential hybridization to high molecular weight proteins by Western blot, we conclude that the antibodies used in this study can recognize specific mucin subtypes in guinea pig airway epithelium and in proteins from GPTE cells. In addition, Muc2 is highly expressed constitutively, modulated by inflammation, and secreted differentially (as compared to Muc5ac) in GPTE cells. This finding contrasts with expression patterns in the airway epithelium of a variety of mammalian species in which only Muc5ac predominates

What is the influence of morphological knowledge in the early stages of reading acquisition among low SES children? A graphical modeling approach

Children from low-SES families are known to show delays in aspects of language development which underpin reading acquisition such as vocabulary and listening comprehension. Research on the development of morphological skills in this group is scarce, and no studies exist in French. The present study investigated the involvement of morphological knowledge in the very early stages of reading acquisition (decoding), before reading comprehension can be reliably assessed. We assessed listening comprehension, receptive vocabulary, phoneme awareness, morphological awareness as well as decoding, word reading and non-verbal IQ in 703 French first-graders from low-SES families after 3 months of formal schooling (November). Awareness of derivational morphology was assessed using three oral tasks: Relationship Judgment (e.g., do these words belong to the same family or not? heat-heater … ham-hammer); Lexical Sentence Completion [e.g., Someone who runs is a …? (runner)]; and Non-lexical Sentence Completion [e.g., Someone who lums is a…? (lummer)]. The tasks differ on implicit/explicit demands and also tap different kinds of morphological knowledge. The Judgement task measures the phonological and semantic properties of the morphological relationship and the Sentence Completion tasks measure knowledge of morphological production rules. Data were processed using a graphical modeling approach which offers key information about how skills known to be involved in learning to read are organized in memory. This modeling approach was therefore useful in revealing a potential network which expresses the conditional dependence structure between skills, after which recursive structural equation modeling was applied to test specific hypotheses. Six main conclusions can be drawn from these analyses about low SES reading acquisition: (1) listening comprehension is at the heart of the reading acquisition process; (2) word reading depends directly on phonemic awareness and indirectly on listening comprehension; (3) decoding depends on word reading; (4) Morphological awareness and vocabulary have an indirect influence on word reading via both listening comprehension and phoneme awareness; (5) the components of morphological awareness assessed by our tasks have independent relationships with listening comprehension; and (6) neither phonemic nor morphological awareness influence vocabulary directly. The implications of these results with regard to early reading acquisition among low SES groups are discussed

Tandem Mass Spectrometry Measurement of the Collision Products of Carbamate Anions Derived from CO2 Capture Sorbents: Paving the Way for Accurate Quantitation

The reaction between CO2 and aqueous amines to produce a charged carbamate product plays a crucial role in post-combustion capture chemistry when primary and secondary amines are used. In this paper, we report the low energy negative-ion CID results for several anionic carbamates derived from primary and secondary amines commonly used as post-combustion capture solvents. The study was performed using the modern equivalent of a triple quadrupole instrument equipped with a T-wave collision cell. Deuterium labeling of 2-aminoethanol (1,1,2,2,-d4-2-aminoethanol) and computations at the M06-2X/6-311++G(d,p) level were used to confirm the identity of the fragmentation products for 2-hydroxyethylcarbamate (derived from 2-aminoethanol), in particular the ions CN−, NCO− and facile neutral losses of CO2 and water; there is precedent for the latter in condensed phase isocyanate chemistry. The fragmentations of 2-hydroxyethylcarbamate were generalized for carbamate anions derived from other capture amines, including ethylenediamine, diethanolamine, and piperazine. We also report unequivocal evidence for the existence of carbamate anions derived from sterically hindered amines (Tris(2-hydroxymethyl)aminomethane and 2-methyl-2-aminopropanol). For the suite of carbamates investigated, diagnostic losses include the decarboxylation product (−CO2, 44 mass units), loss of 46 mass units and the fragments NCO− (m/z 42) and CN− (m/z 26). We also report low energy CID results for the dicarbamate dianion (−O2CNHC2H4NHCO2−) commonly encountered in CO2 capture solution utilizing ethylenediamine. Finally, we demonstrate a promising ion chromatography-MS based procedure for the separation and quantitation of aqueous anionic carbamates, which is based on the reported CID findings. The availability of accurate quantitation methods for ionic CO2 capture products could lead to dynamic operational tuning of CO2 capture-plants and, thus, cost-savings via real-time manipulation of solvent regeneration energies